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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha : |
21/02/2014 |
Actualizado : |
05/11/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
FRAGA, M.; FERNÁNDEZ, S.; CAJARVILLE, C.; MARTÍNEZ, M.; ABIN-CARRIQUIRY, J.A.; ZUNINO, P. |
Afiliación : |
MARTIN FRAGA COTELO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./Departamento de Microbiología Instituto de Investigaciones Biológicas Clemente Estable Montevideo Uruguay. |
Título : |
In vitro modulation of rumen microbiota and fermentation by native microorganisms isolated from the rumen of a fed-exclusively-on-pasture bovine. |
Fecha de publicación : |
2015 |
Fuente / Imprenta : |
Annals of Microbiology, v. 65 n. 4, p. 2355-2362, 2015. |
Idioma : |
Inglés |
Notas : |
Article history: Received: 6 January 2015 /Accepted: 17 March 2015. |
Contenido : |
Abstract:
In order to increase productive yields, modulation of rumen fermentation has been a concern in economically relevant species. The ban of antibiotics has driven attention into alternative strategies to modulate ruminal fermentation. Among these, the use of probiotics appears as an interesting approach. The objective of this work was to assess the potential of native bacteria isolated from the rumen of a fed-on-template-pasture cow to modulate fermentation in vitro and to influence the microbiota structure. Seven native ruminal bacteria strains were used in an in vitro gas production experiment. Fermentation dynamics were evaluated, and volatile fatty acids (VFA) and methane were quantified by high-performance liquid chromatography (HPLC) and gas chromatography (GC), respectively. Microbiota structure was assessed by pyrosequencing and methanogens were quantified by quantitative PCR (qPCR). Added strains modulated fermentation dynamics and VFA synthesis. Neither the general structure of the fermenters microbiota, numbers of methanogenic microorganisms nor methane production were altered by added bacteria. However, addition of two strains reduced the volume of gas produced from soluble carbohydrates, while one of them reduced the ratio of gas production in this phase; this was supported by a VFA concentration diminution (4 h incubation) in almost every treatment. Gas produced by fermentation of non-soluble carbohydrates and its fermentation ratio were enhanced by several strains. Also, the abundances of Lachnospiraceae, Veillonellaceae, Rikenellaceae and Succinivibrionaceae were affected by strain supplementation. Modulation of fermentation by selected ruminal native bacteria was achieved, probably enhancing the fermentation of non-soluble carbohydrates. This study represents a new approach in the knowledge related to the use of probiotics in ruminants. MenosAbstract:
In order to increase productive yields, modulation of rumen fermentation has been a concern in economically relevant species. The ban of antibiotics has driven attention into alternative strategies to modulate ruminal fermentation. Among these, the use of probiotics appears as an interesting approach. The objective of this work was to assess the potential of native bacteria isolated from the rumen of a fed-on-template-pasture cow to modulate fermentation in vitro and to influence the microbiota structure. Seven native ruminal bacteria strains were used in an in vitro gas production experiment. Fermentation dynamics were evaluated, and volatile fatty acids (VFA) and methane were quantified by high-performance liquid chromatography (HPLC) and gas chromatography (GC), respectively. Microbiota structure was assessed by pyrosequencing and methanogens were quantified by quantitative PCR (qPCR). Added strains modulated fermentation dynamics and VFA synthesis. Neither the general structure of the fermenters microbiota, numbers of methanogenic microorganisms nor methane production were altered by added bacteria. However, addition of two strains reduced the volume of gas produced from soluble carbohydrates, while one of them reduced the ratio of gas production in this phase; this was supported by a VFA concentration diminution (4 h incubation) in almost every treatment. Gas produced by fermentation of non-soluble carbohydrates and its fermentation ratio were enhanced by seve... Presentar Todo |
Palabras claves : |
FERMENTATION MODULATION; IN VITRO GAS PRODUCTION; MICROBIOTA RUMINAL; NATIVE RUMINAL MICROORGANISMS; PROBIÓTICOS; PROBIOTICS; RUMEN MICROBIOTA. |
Thesagro : |
PRODUCCIÓN ANIMAL. |
Asunto categoría : |
-- |
Marc : |
LEADER 02838naa a2200289 a 4500 001 1049916 005 2019-11-05 008 2015 bl uuuu u00u1 u #d 100 1 $aFRAGA, M. 245 $aIn vitro modulation of rumen microbiota and fermentation by native microorganisms isolated from the rumen of a fed-exclusively-on-pasture bovine. 260 $c2015 500 $aArticle history: Received: 6 January 2015 /Accepted: 17 March 2015. 520 $aAbstract: In order to increase productive yields, modulation of rumen fermentation has been a concern in economically relevant species. The ban of antibiotics has driven attention into alternative strategies to modulate ruminal fermentation. Among these, the use of probiotics appears as an interesting approach. The objective of this work was to assess the potential of native bacteria isolated from the rumen of a fed-on-template-pasture cow to modulate fermentation in vitro and to influence the microbiota structure. Seven native ruminal bacteria strains were used in an in vitro gas production experiment. Fermentation dynamics were evaluated, and volatile fatty acids (VFA) and methane were quantified by high-performance liquid chromatography (HPLC) and gas chromatography (GC), respectively. Microbiota structure was assessed by pyrosequencing and methanogens were quantified by quantitative PCR (qPCR). Added strains modulated fermentation dynamics and VFA synthesis. Neither the general structure of the fermenters microbiota, numbers of methanogenic microorganisms nor methane production were altered by added bacteria. However, addition of two strains reduced the volume of gas produced from soluble carbohydrates, while one of them reduced the ratio of gas production in this phase; this was supported by a VFA concentration diminution (4 h incubation) in almost every treatment. Gas produced by fermentation of non-soluble carbohydrates and its fermentation ratio were enhanced by several strains. Also, the abundances of Lachnospiraceae, Veillonellaceae, Rikenellaceae and Succinivibrionaceae were affected by strain supplementation. Modulation of fermentation by selected ruminal native bacteria was achieved, probably enhancing the fermentation of non-soluble carbohydrates. This study represents a new approach in the knowledge related to the use of probiotics in ruminants. 650 $aPRODUCCIÓN ANIMAL 653 $aFERMENTATION MODULATION 653 $aIN VITRO GAS PRODUCTION 653 $aMICROBIOTA RUMINAL 653 $aNATIVE RUMINAL MICROORGANISMS 653 $aPROBIÓTICOS 653 $aPROBIOTICS 653 $aRUMEN MICROBIOTA 700 1 $aFERNÁNDEZ, S. 700 1 $aCAJARVILLE, C. 700 1 $aMARTÍNEZ, M. 700 1 $aABIN-CARRIQUIRY, J.A. 700 1 $aZUNINO, P. 773 $tAnnals of Microbiology$gv. 65 n. 4, p. 2355-2362, 2015.
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INIA La Estanzuela (LE) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
21/02/2014 |
Actualizado : |
02/06/2017 |
Tipo de producción científica : |
Serie FPTA |
Autor : |
BREIJO, M.; BOLATTO, C.; FERNÁNDEZ, C. |
Afiliación : |
MARTÍN BREIJO, Universidad de la República (UdelaR)/ Facultad de Medicina; CARMEN BOLETTO, Universidad de la República (UdelaR)/ Facultad de Medicina; CECILIA FERNÁNDEZ, Universidad de la República (UdelaR)/ Facultad de Química. |
Título : |
Mosca de los cuernos: estudios hacia el desarrollo de vacunas como métodos alternativos de control |
Fecha de publicación : |
2013 |
Fuente / Imprenta : |
Montevideo (UY): INIA, 2013. |
Páginas : |
16 p. |
Serie : |
(Serie FPTA-INIA; 49) |
ISSN : |
1688-924X |
Idioma : |
Español |
Notas : |
Proyecto FPTA 224. Identificación y obtención de antígeno de la saliva de mosca de los cuernos (Haematobia irritans irritans). Evaluación de los mismos como blancos de vacunas. Período de Ejecución: Feb. 2007-Feb. 2010.
Personal contratado: Lucía Pastro; Malvina Ocampo, Sergio Rocha, Valeria Anastasia, Mariana Balducci, Mariana Curto. |
Palabras claves : |
MOSCA DE LOS CUERNOS. |
Thesagro : |
ENFERMEDADES DE LOS ANIMALES; ENFERMEDADES PARASITARIAS. |
Asunto categoría : |
-- |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/1371/1/18429161213110056.pdf
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Marc : |
LEADER 00962nam a2200205 a 4500 001 1003676 005 2017-06-02 008 2013 bl uuuu u00u1 u #d 022 $a1688-924X 100 1 $aBREIJO, M. 245 $aMosca de los cuernos$bestudios hacia el desarrollo de vacunas como métodos alternativos de control$h[electronic resource] 260 $aMontevideo (UY): INIA$c2013 300 $a16 p. 490 $a(Serie FPTA-INIA; 49) 500 $aProyecto FPTA 224. Identificación y obtención de antígeno de la saliva de mosca de los cuernos (Haematobia irritans irritans). Evaluación de los mismos como blancos de vacunas. Período de Ejecución: Feb. 2007-Feb. 2010. Personal contratado: Lucía Pastro; Malvina Ocampo, Sergio Rocha, Valeria Anastasia, Mariana Balducci, Mariana Curto. 650 $aENFERMEDADES DE LOS ANIMALES 650 $aENFERMEDADES PARASITARIAS 653 $aMOSCA DE LOS CUERNOS 700 1 $aBOLATTO, C. 700 1 $aFERNÁNDEZ, C.
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